role

Liver cell Scientist
Quality Manager

qualifications

THE UNIVERSITY OF SHEFFIELD, MSc Molecular and Genetic Medicine
THE UNIVERSITY OF LEEDS BSc Honours degree in Medical Microbiology.

research project title(s)

Improving quality of human hepatocytes

core expertise

Human hepatocyte isolation and preparation under GMP conditions for clinical use
Cryopreservation of Human hepatocyte
Running of a GMP laboratory
– GMP Regulation HTA/MHRA
– Quality management
– Environmental monitoring
– Q-pulse

contact

Sharon.Lehec@nhs.net
Tel +44 203 299 1545

Main publications

Alginate microencapsulated hepatocytes optimised for transplantation in acute liver failure.
Jitraruch S, Dhawan A, Hughes RD, Filippi C, Soong D, Philippeos C, Lehec SC, Heaton ND, Longhi MS, Mitry RR.
PLoS One. 2014 Dec 1;9(12):e113609. doi: 10.1371/journal.pone.0113609. eCollection 2014.

Experience of microbiological screening of human hepatocytes for clinical transplantation.
Lehec SC, Hughes RD, Mitry RR, Graver MA, Verma A, Wade JJ, Dhawan A. Cell Transplant. 2009;18(8):941-7. doi: 10.3727/096368909X471323. Epub 2009 May 6.

N-acetylcysteine improves the viability of human hepatocytes isolated from severely steatotic donor liver tissue.
Sagias FG, Mitry RR, Hughes RD, Lehec SC, Patel AG, Rela M, Mieli-Vergani G, Heaton ND, Dhawan A. Cell Transplant. 2010;19(11):1487-92. doi: 10.3727/096368910X514620. Epub 2010 Jun 29.

Optimization of the cryopreservation and thawing protocol for human hepatocytes for use in cell transplantation.
Terry C, Dhawan A, Mitry RR, Lehec SC, Hughes RD. Liver Transpl. 2010 Feb;16(2):229-37. doi: 10.1002/lt.21983.

Liver after hepatocyte transplantation for liver-based metabolic disorders in children.
Quaglia A, Lehec SC, Hughes RD, Mitry RR, Knisely AS, Devereaux S, Richards J, Rela M, Heaton ND, Portmann BC, Dhawan A. Cell Transplant. 2008;17(12):1403-14.

Isolation of hepatocytes from livers from non-heart-beating donors for cell transplantation.
Hughes RD, Mitry RR, Dhawan A, Lehec SC, Girlanda R, Rela M, Heaton ND, Muiesan P. Liver Transpl. 2006 May;12(5):713-7.

Hepatocyte transplantation for inherited factor VII deficiency.
Dhawan A, Mitry RR, Hughes RD, Lehec S, Terry C, Bansal S, Arya R, Wade JJ, Verma A, Heaton ND, Rela M, Mieli-Vergani G. Transplantation. 2004 Dec 27;78(12):1812-4.

One liver, three recipients: segment IV from split-liver procedures as a source of hepatocytes for cell transplantation.
Mitry RR, Dhawan A, Hughes RD, Bansal S, Lehec S, Terry C, Heaton ND, Karani JB, Mieli-Vergani G, Rela M. Transplantation. 2004 May 27;77(10):1614-6.

Chapters

Cryopreservation of human hepatocytes for clinical use.
Mitry RR, Lehec SC, Hughes RD. Methods Mol Biol. 2010;640:107-13. doi: 10.1007/978-1-60761-688-7_5.

Microbiological monitoring of hepatocyte isolation in the GMP laboratory.
Lehec SC. Methods Mol Biol. 2009;481:221-7. doi: 10.1007/978-1-59745-201-4_18. Review.

 

[PubMed list  – full list of publications]