Ragai R. Mitry

roleRagai

Head of Liver Cell Production and Research
Reader in Hepatocyte Transplantation

qualifications

BSc(Hons) in Biochemistry, Polytechnic of North London, London.
Postgraduate Diploma in Computing, University of North London, London.
Diploma of Imperial College in Gene Therapy (DIC), Imperial College, London.
PhD, Imperial College, London.

research project title(s)

Optimisation of protocols for isolation and preparation of clinical grade human hepatocytes
Optimisation of protocols for production of clinical grade alginate-encapsulated hepatocytes (hepatocyte  microbeads)
Study the effects of human serum obtained from acute liver failure patients on hepatocyte microbeads function and activity

core expertise

Human hepatocyte isolation and preparation under GMP conditions for clinical use
Development of new techniques for human hepatocyte transplantation including hepatocyte microbeads for treatment of children with acute liver failure
Alginate encapsulation of mammalian cells
Human hepatocyte culture, and function and activity assays

contact

ragai.mitry@kcl.ac.uk
Tel: +44 20 32991545

publications

Original Research Papers

Alginate microencapsulated hepatocytes optimised for transplantation in acute liver failure.
Jitraruch S, Dhawan A, Hughes RD, Filippi C, Soong D, Philippeos C, Lehec SC, Heaton ND, Longhi MS, Mitry RR.
PLoS One. 2014 Dec 1;9(12):e113609. doi: 10.1371/journal.pone.0113609. eCollection 2014. [pubmed]

Puppi J, Modo M, Dhawan A, Lehec SC, Mitry RR, and Hughes RD. (2011). Ex Vivo magnetic resonance imaging of transplanted hepatocytes in a rat model of acute liver failure. Cell Transplantation 20(6), 963-975.[Joint senior authors: Mitry and Hughes]

Possamai LA, McPhail MJ, Quaglia A, Zingarella V, Abeles RD, Tidswell R, Puthucheary Z, Rawal J, Karvellas CJ, Leslie EM, Hughes RD, Ma Y, Jassem W, Shawcross DL, Bernal W, Dhawan A, Heaton ND, Thurs MR, Wendon JA, Mitry RR, and Antoniades CG. (2013). Character and temporal evolution of apoptosis in acetaminophen-induced acute liver failure. Critical Care Medicine 41(11), 2543-2550. [Joint senior authors: Wendon, Mitry and Antoniades]

Fitzpatrick E, Dew TK, Quaglia A, Sherwood RA, Mitry RR, and Dhawan A. (2013). Analysis of adipokine concentrations in paediatric non-alcoholic fatty liver disease. Pediatric Obesity 7(6), 471-479.

Ho CM, Dhawan A, Hughes RD, Lehec SC, Puppi J, Philippeos C, Lee PH, and Mitry RR. (2012). Use of indocyanine green for functional assessment of human hepatocytes for transplantation. Asian Journal of Surgery 35(1), 9-15.

Puppi J, Mitry RR, Modo M, Dhawan A, Raja K, and Robin D. Hughes. (2011). Use of a Clinically-Approved Iron Oxide MRI Contrast Agent to Label Human Hepatocytes. Cell Transplantation 20(6), 963-975.

Sagias FG, Mitry RR, Hughes RD, Lehec SC, Patel AG, Mieli-Vergani G, Rela M, Heaton ND, and Dhawan A. (2010). N-acetylcysteine markedly improves the function of human hepatocytes isolated from severely steatotic donor liver tissue – Potential to increase the liver donor pool. Cell Transplantation 19, 1487-1492.

Mitry RR, Bansal S, Hughes RD, Mieli-Vergani G, and Dhawan A. (2009). In vitro effects of sera from children with acute liver failure on metabolic and synthetic activity of cryopreserved human hepatocytes.  Journal of Pediatric Gastroenterology and Nutrition 48(5), 604-7.

Aw MM, Mitry RR, Hughes RD, and Dhawan A.  (2006). Serum hepatocyte growth factor and vascular endothelial growth factor in children with acute liver failure.  Journal of Pediatric Gastroenterology and Nutrition 44(2), 224-227.

Mitry RR, Hughes RD, Bansal S, Lehec SC, Wendon JA, and Dhawan A. (2005). Effects of Serum from Patients with Acute Liver Failure due to Paracetamol Overdose on Human Hepatocytes In Vitro.  Transplantation Proceedings 37, 2391-2394.

Dhawan A, Mitry RR, Hughes RD, Lehec S, Terry C, Bansal S, Arya R, Wade JJ, Verma A, Heaton ND, Rela M, and Mieli-Vergani G. (2004). Hepatocyte transplantation for inherited factor VII deficiency.  Transplantation 78(12), 1812-1814.

Mitry RR, Dhawan A, Hughes RD, Bansal S, Lehec S, Terry C, Heaton ND, Karani JB, Mieli-Vergani G, and Rela M.  (2004). One liver, three recipients – segment IV from split liver procedures as a source of hepatocytes for cell transplantation.  Transplantation 77(10), 1614-1616.

Mitry RR, Hughes RD, Aw MM, Terry C, Mieli-Vergani G, Girlanda G, Muiesan P, Rela M, Heaton ND, and Dhawan A. (2003). Human Hepatocyte Isolation and Relationship of Cell Viability to Early Graft Function.  Cell Transplantation 12(1), 69-74.

Books

Basic Introduction to Cell Culture. Mitry RR, and Hughes RD, eds. (3rd edition). Methods in Molecular Medicine: Human Cell Culture Protocols. 2011. Humana Press Inc.: New Jersey.

Review articles, book chapters and other output

Ryan JM, Tranah T, Mitry RR, Wendon JA, and Shawcross DL. (2013). Acute liver failure and the brain: a look through the crystal ball. Metabolic Brain Disease 28(1), 7-10.

Jitraruch S, Qin H, Dhawan A, Hughes RD, Lehec SC, and Mitry RR. (2012). Hepatocyte Transplantation, ‘Omics’ and Gene Therapy in the management of Liver Diseases. In: Dhawan A, ed. (Vol. 16). Concise Pediatric and Adolescent Hepatology. Karger AG: Basel (Switzerland), 240-256. [book cover designed by Dr. RR Mitry]

Mitry RR, Hughes RD, and Dhawan A. (2011). Concise Review: Hepatocyte transplantation – from bench to bedside. Journal of Clinical and Experimental Hepatology 1,109–114. [invited review]

Philippeos C, Hughes RD, Dhawan A, and Mitry RR. (2011). Basic Introduction to Cell Culture. In: Mitry RR, and Hughes RD, eds. (3rd edition). Methods in Molecular Medicine: Human Cell Culture Protocols. Humana Press Inc.: New York, 1-13.

Mustafa A, Cenayko C, Mitry RR, and Quaglia A. (2011). Laser Microdissection Applications to Live Cells From Tissue Culture. In: Mitry RR, and Hughes RD, eds. (3rd edition).  Methods in Molecular Medicine: Human Cell Culture Protocols. Humana Press Inc.: New York, 385-392.

Dhawan A, Puppi J, Hughes RD, and Mitry RR. (2010). Hepatocyte transplantation: update and challenges ahead. Nature Reviews Gastroenterology and Hepatology 7(5), 288-298. [invited review]

Mitry RR, Lehec SC, and Hughes RD. (2010). Cryopreservation of Human Hepatocytes for Clinical Use. In: Maurel P, ed. Methods in Molecular Biology: Hepatocytes. Humana Press Inc.: New Jersey, 107-114.

Mitry RR. (2009). Isolation of human hepatocytes. In: Dhawan A and Hughes RD, eds. Methods in Molecular Biology: Hepatocyte Transplantation. Humana Press Inc.: New Jersey, 17-23.

Modo M., Meade TJ, and Mitry RR. (2009). Liver cell labelling with MRI contrast agents. In: Dhawan A and Hughes RD, eds. Methods in Molecular Biology: Hepatocyte Transplantation. Humana Press Inc.: New Jersey, 207-219.

Mitry RR, De Bruyne R, Quaglia A, Hughes RD, and Dhawan A.  (2007). Non-invasive diagnosis of non-alcoholic fatty liver disease using serum biomarkers.  Hepatology 46(6), 2047-2048. [letter to the Editor]

Dhawan A, Mitry RR, and Hughes RD. (2005). Hepatocyte Transplantation for metabolic disorders, experience at King’s College Hospital and review of literature. Acta Gastroenterologica Belgica 68(4), 457-460. [invited review]

[Full PubMed list]

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