Anil Chandrashekran

roleAnilC

Post-Doctoral Research Associate

qualifications

Ph.D.      Genetics            Imperial College London, University of London (2003)
M Sc.      Genetics            University of Malaya, Malaysia  (1995)
B Sc. (Hons) Genetics    University of Malaya, Malaysia  (1991)

research project title(s)

Developing a 3-Dimensional hepatocyte-stem cell derived organoid system for the treatment of acute paediatric liver failure

core expertise

Molecular Biology
Gene delivery into primary cells, stem cells and cell lines using retroviral vectors
Gene silencing and editing of primary cells
Male germ cells and transgenic animal development

contact

anil.chandrashekran@kcl.ac.uk
Tel: +44 203 299 1545

main publications

Khan MK, Chandrashekran A, Smith RK, Dudhia J. Immunophenotypic characterisation of ovine mesenchymal stem cells, Cytometry. 2016 May;89(5):443-50. doi: 10.1002/cyto.a.22849. Epub 2016 Apr 14

Tan GC, Chan E, Molnar A, Sarkar R, Alexieva D, Robinson S, Isa I, Zhang S, Ellis P, Langford C, Guillot P, Fisk NM, Chandrashekran A, Castellano L, Meister G, Winston RM, Cui W, Baulcombe DC, Dibb NJ. 5’ isomiR variation is of functional and evolutionary importance. Nucleic Acids Res. 2014 Oct 1;42(14):9424-35. doi: 10.1093/nar/gku656. Epub 2014 Jul 23

Chandrashekran A, Isa I, Dudhia J,Thrasher AJ, Dibb NJ, Casimir C, Readhead C, Winston R. Lentiviral vector transduction of spermatozoa as a tool for the study of early development. FEBS Open Bio. 2014 Mar 3;4:266-75. DOI: 10.1016/j.fob.2014.02.008. eCollection 2014

Witney TH, Carroll L, Alam IS, Chandrashekran A, Nguyen QD, Sala R, Harris R, Deberardinis RJ, Agarwal R, Aboagye EO. A novel radiotracer to image glycogen metabolism in tumors by positron emission tomography. CANCER RES. 2014 MAR 1;74(5):1319-28. DOI: 10.1158/0008-5472.CAN-13-2768

Chandrashekran A, Sarkar R, Thrasher A, Fraser SE, Dibb N, Casimir C, Winston R, Readhead C. Efficient generation of transgenic mice by lentivirus-mediated modification of spermatozoa. FASEB J.2014 Feb;28(2):569-76. doi: 10.1096/fj.13-233999. Epub 2013 Dec 2

Lavial F, Bessonnard S, Ohnishi Y, Tsumura A, Chandrashekran A, Fenwick MA, Tomaz RA, Hosokawa H, Nakayama T, Chambers I, Hiiragi T, Chazaud C, Azuara V. Bmi1 facilitates primitive endoderm formation by stabilizing Gata6 during early mouse development. Genes and Development.2012 Jul 1;26(13):1445-58. doi: 10.1101/gad.188193.112. Epub 2012 Jun 19

Alder O, Lavial F, Helness A, Brookes E, Pinho S, Chandrashekran A, Arnaud P, Pombo A, O’Neill L, Azuara V.Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment. Development.2010 Aug 1;137(15):2483-92. doi: 10.1242/dev.048363. Epub 2010 Jun 23

Chandrashekran A, Gordon MY, Casimir C. Targeted retroviral transduction of kit+ haematopoietic cells using novel ligand-display technology. Blood.2004 Nov 1;104(9):2697-703. Epub 2004 Jul 15

Chandrashekran A, Gordon MY, Darling D, Farzaneh F, Casimir C. Growth factor displayed on the surface of retroviral particles without manipulation of envelope proteins is biologically active and can enhance transduction. J. of Gene Medicine.2004 Nov;6(11):1189-96

Book Chapter

Chandrashekran A, Casimir C, Dibb N, Readhead C, Winston R. Generating transgenic mice by lentiviral transduction of spermatozoa followed by in vitro fertilization and embryo transfer. In Lentiviral vectors and exosomes as gene and protein delivery tools. Methods in Molecular Biology (MMB) 2016: 1448:95-106. doi: 10.1007/978-1-4939-3753-0_8.

Howe SJ, Chandrashekran A. Principles of retrovirus vector design and production. In Prenatal Gene Therapy. Concepts, Methods, and Protocols. Methods Mol Biol.2012;891:85-107. doi: 10.1007/978-1-61779-873-3_5